RNF168 antibody (C-Term)

Catalog No. ABIN500624

Product Details anti-RNF168 Antibody

Target: RNF168 (Ring Finger Protein 168 (RNF168))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF168 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects RNF168.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogen: RNF168 antibody was raised against an 18 amino acid peptide from near the carboxy terminus of human RNF168.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-RNF168 antibody (ABIN500624)

Li, Guan, Huang, Hu, Zheng: "RNF168-mediated H2A neddylation antagonizes ubiquitylation of H2A and regulates DNA damage repair." in: Journal of cell science, Vol. 127, Issue Pt 10, pp. 2238-48, (2014) (PubMed).

Target Details for RNF168

Target: RNF168 (Ring Finger Protein 168 (RNF168))

Alternative Name: RNF168 (RNF168 Products)

Synonyms: 3110001H15Rik antibody, hRNF168 antibody, ring finger protein 168 antibody, ring finger protein 168, E3 ubiquitin protein ligase L homeolog antibody, Rnf168 antibody, rnf168.L antibody, RNF168 antibody

Background: RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.Synonyms: E3 ubiquitin-protein ligase RNF168, RING finger protein 168

Gene ID: 165918

NCBI Accession: NP_689830

UniProt: Q8IYW5

Pathways: Production of Molecular Mediator of Immune Response