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Details for Product No. ABIN500784

Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 222-237), (C-Term) antibody

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Antigen
Synonyms Smac, DFNA64, SMAC, DBO, XSmac, smac, smac3, diablo-s
Epitope
»Alternatives AA 222-237, C-Term
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN500784
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
Isotype IgG
Specificity This antibody detects DIABLO / SMAC at C-term. Species Reactivity: Tested: Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name DIABLO / SMAC
Background The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).
Alternate names: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 66593
NCBI Accession AF203914
UniProt Q9JIQ3
Application Notes ELISA. Western blot: 0.5 to 1 μg/ml, 25 kDa band can be detected. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

Alternatives for antigen "Second Mitochondria-Derived Activator of Caspase (DIABLO)", type "Antibodies"
Hosts (119), (26), (5)
Reactivities (136), (54), (48), (14), (14), (13), (13), (2), (2), (1), (1), (1)
Applications (136), (43), (41), (35), (25), (10), (6), (6), (6), (5), (4), (3), (3), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (27), (10), (7), (6), (5), (4), (3), (2), (2), (2), (2), (1)
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