Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 222-237), (C-Term) antibody

Details for Product No. ABIN500784
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Antigen
Synonyms smac, smac3, diablo-s, 0610041G12Rik, 1700006L01Rik, AU040403, Smac, DFNA64, SMAC, DBO, XSmac
Epitope
AA 222-237, C-Term
(32), (20), (11), (10), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(137), (56), (45), (15), (15), (14), (13), (2), (1)
Host
Rabbit
(121), (23), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(130), (48), (43), (34), (17), (16), (10), (6), (6), (5), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN500784
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Immunogen Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
Isotype IgG
Specificity This antibody detects DIABLO / SMAC at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name DIABLO / SMAC
Background The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 66593
UniProt Q9JIQ3
Application Notes ELISA. Western blot: 0.5 to 1 μg/mL, 25 kDa band can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Background publications Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

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