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Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 222-237), (C-Term) antibody

Details for Product No. ABIN500784, Supplier: Log in to see
Antigen
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
Alternatives
anti-Human Second Mitochondria-Derived Activator of Caspase antibody for Western Blotting
Epitope
AA 222-237, C-Term
31
16
12
11
7
5
4
4
4
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
134
54
34
4
4
3
2
1
1
Host
Rabbit
114
29
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
139
57
56
31
21
10
8
6
4
3
2
2
2
1
1
1
1
1
Supplier
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Immunogen Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
Isotype IgG
Specificity This antibody detects DIABLO / SMAC at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 66593
UniProt Q9JIQ3
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA. Western blot: 0.5 to 1 μg/mL, 25 kDa band can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 222-237), (C-Term) antibody (ABIN500784) Immunohistochemical staining of mouse spleen tissue using AP30815PU-N at 2 μg/ml.
Western Blotting (WB) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 222-237), (C-Term) antibody (ABIN500784) Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or pres...
Background publications Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).