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STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (C-Term)

Details for Product anti-STAT1 Antibody No. ABIN500815, Supplier: Log in to see
Antigen
  • IMUP
  • H2RSP
  • IMUP-1
  • IMUP-2
  • 2010005J02Rik
  • AA408197
  • DD6G4-4
  • CANDF7
  • ISGF-3
  • STAT91
  • STAT4
  • XStat1
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
Alternatives
anti-Human STAT1 antibody for Immunoprecipitation
Epitope
C-Term
75
73
68
18
18
14
11
8
7
6
6
5
5
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
433
212
171
26
16
15
15
14
7
5
4
2
2
2
2
2
2
1
1
Host
Rabbit
354
101
3
3
1
Clonality
Polyclonal
Conjugate
This STAT1 antibody is un-conjugated
10
9
9
7
7
7
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
376
156
136
88
62
47
33
18
18
9
9
6
5
2
2
1
Supplier
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Immunogen STAT1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human STAT1 alpha. Remarks: The sequences differ from the murine corresponding sequences by four amino acids.
Isotype IgG
Specificity This antibody detects STAT1 alpha (C-term) at a 91 kDa band. No reaction to the 84 kDa STAT1a.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification DEAE purified
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta which are encoded by the same gene with splicing variant.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Gene ID 6772
NCBI Accession NP_009330
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway
Application Notes ELISA. Western blot: 1: 1000 - 1: 2000. Immunoprecipitation: 2 - 4 μg. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (C-Term) (ABIN500815) Immunocytochemistry of STAT1 alpha in HeLa cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-STAT1 antibody (Signal Transducer and Activator of Transcription 1, 91kDa) (C-Term) (ABIN500815) Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J),...
Background publications Leonard, OShea: "Jaks and STATs: biological implications." in: Annual review of immunology, Vol. 16, pp. 293-322, 1998 (PubMed).

Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997 (PubMed).

Schindler, Darnell: "Transcriptional responses to polypeptide ligands: the JAK-STAT pathway." in: Annual review of biochemistry, Vol. 64, pp. 621-51, 1995 (PubMed).

Schindler, Fu, Improta et al.: "Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7836-9, 1992 (PubMed).