ANO1 antibody (N-Term)

Catalog No. ABIN500970

Product Details anti-ANO1 Antibody

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: At least three isoforms are known to exist. This antibody will not cross-react with TMEM16B.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Peptide affinity chromatography

Immunogen: 17 amino acid peptide from near the amino terminus of Human TMEM16A

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: ANO1 / DOG1 (ANO1 Products)

Synonyms: DOG1 antibody, ORAOV2 antibody, TAOS2 antibody, TMEM16A antibody, Tmem16a antibody, anoctamin 1 antibody, anoctamin 1, calcium activated chloride channel antibody, Ano1 antibody, ANO1 antibody

Background: Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC's include the transmembrane proteins TMEM16A and TMEM16B. TMEM16A is expressed in epithelial cells of the kidney and lung, pancreas, and sensory neurons and its mRNA is seen in foregut, airway epithelia, and tracheal smooth muscle, mice lacking TMEM16A fail to survive past ten days and show aerophagia and little weight gain. TMEM16A is also overexpressed or amplified in multiple cancers associated with poor survival such as oral cancers and gastrointestinal stromal tumors, suggesting the development of CaCC modulators may be a viable therapeutic strategy.Synonyms: Anoctamin-1, DOG-1, Discovered on gastrointestinal stromal tumors protein 1, ORAOV2, Oral cancer overexpressed protein 2, TAOS2, TMEM16A, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2

Gene ID: 55107

NCBI Accession: NP_060513