Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (N-Term) antibody

Details for Product No. ABIN501014
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Antigen
Synonyms FRAP1, FRAP, FRAP2, RAFT1, RAPT1, 2610315D21Rik, AI327068, Frap1, flat, frap1, tor, wu:fc22h08
Epitope
N-Term
(48), (23), (22), (12), (12), (11), (8), (7), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(263), (153), (147), (62), (36), (24), (2), (2), (2), (1)
Host
Rabbit
(261), (13), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(160), (79), (78), (76), (50), (41), (26), (26), (4), (3), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN501014
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Immunogen TOR antibody was raised against a 15 amino acid peptide from near the amino terminus of human TOR.
Isotype IgG
Specificity This antibody detects mTOR / FRAP1.
Purification Peptide affinity chromatography
Alternative Name mTOR / FRAP1
Background The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin (2,3). Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 4). TOR is normally associated with the regulatory proteins RAPTOR and GbetaL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis (4).
Alternate names: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
Application Notes ELISA. Western blot: 1 μg/ml. Immunoflourescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Sabatini, Erdjument-Bromage, Lui et al.: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994 (PubMed).

Cardenas, Zhu, Heitman: "Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin." in: Current opinion in nephrology and hypertension, Vol. 4, Issue 6, pp. 472-7, 1996 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Fingar, Blenis: "Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression." in: Oncogene, Vol. 23, Issue 18, pp. 3151-71, 2004 (PubMed).

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