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MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (N-Term)

Details for Product anti-FRAP1 Antibody No. ABIN501014, Supplier: Log in to see
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
Alternatives
anti-Human MTOR antibody for Western Blotting
Epitope
N-Term
65
25
24
23
21
20
14
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13
8
7
6
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human, Mouse (Murine)
335
159
148
7
2
2
2
1
1
Host
Rabbit
287
47
2
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
7
7
7
5
4
4
4
4
4
4
4
4
4
3
3
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
215
110
87
60
51
45
32
19
8
7
6
3
2
2
2
2
2
1
Supplier
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Immunogen TOR antibody was raised against a 15 amino acid peptide from near the amino terminus of human TOR.
Isotype IgG
Specificity This antibody detects mTOR / FRAP1.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Alternative Name mTOR / FRAP1 (FRAP1 Antibody Abstract)
Background The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin (2,3). Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 4). TOR is normally associated with the regulatory proteins RAPTOR and GbetaL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis (4).Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA. Western blot: 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (N-Term) (ABIN501014) Immunocytochemistry of TOR in L1210 cells with this product at 2 μg/ml.
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (N-Term) (ABIN501014) Western blot analysis of TOR in L1210 cell lysate with this product at (A) 1 and (B) ...
Background publications Fingar, Blenis: "Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression." in: Oncogene, Vol. 23, Issue 18, pp. 3151-71, 2004 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Cardenas, Zhu, Heitman: "Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin." in: Current opinion in nephrology and hypertension, Vol. 4, Issue 6, pp. 472-7, 1996 (PubMed).

Sabatini, Erdjument-Bromage, Lui et al.: "RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs." in: Cell, Vol. 78, Issue 1, pp. 35-43, 1994 (PubMed).