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Tripartite Motif Containing 25 (TRIM25) (N-Term) antibody

Details for Product No. ABIN501046, Supplier: Log in to see
Antigen
  • zgc:55773
  • MGC130751
  • EFP
  • RNF147
  • Z147
  • ZNF147
  • AA960166
  • AL022677
  • Zfp147
  • Efp
  • Znf147
Epitope
N-Term
18
15
14
14
12
11
10
5
5
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
119
31
18
1
1
1
1
1
1
Host
Rabbit
106
13
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
87
50
24
20
16
15
13
5
2
2
2
Supplier
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Immunogen TRIM25 antibody was raised against a 15 amino acid peptide from near the amino terminus of human TRIM25.
Isotype IgG
Specificity This antibody detects TRIM25 / RNF147 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Peptide affinity chromatography
Alternative Name TRIM25 / RNF147 (TRIM25 Antibody Abstract)
Background TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.Synonyms: EFP, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, ZNF147, Zinc finger protein 147
Gene ID 7706
NCBI Accession NP_005073
Application Notes ELISA. Western blot. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-Tripartite Motif Containing 25 (TRIM25) (N-Term) antibody (ABIN501046) Immunocytochemistry of TRIM25 in HeLa cells with this product at 5 μg/ml.
Western Blotting (WB) image for anti-Tripartite Motif Containing 25 (TRIM25) (N-Term) antibody (ABIN501046) Western blot analysis of TRIM25 in human thymus tissue lysate in (A) the absence and ...
Background publications Urano, Saito, Tsukui et al.: "Efp targets 14-3-3 sigma for proteolysis and promotes breast tumour growth." in: Nature, Vol. 417, Issue 6891, pp. 871-5, 2002 (PubMed).

Thomson, Ali, Pickles et al.: "Analysis of estrogen-responsive finger protein expression in benign and malignant human breast." in: International journal of cancer. Journal international du cancer, Vol. 91, Issue 2, pp. 152-8, 2001 (PubMed).

Orimo, Inoue, Minowa et al.: "Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 21, pp. 12027-32, 1999 (PubMed).

Inoue, Orimo, Hosoi et al.: "Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 23, pp. 11117-21, 1994 (PubMed).