Tuberous Sclerosis 2 (TSC2) (C-Term) antibody

Details for Product No. ABIN501073
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Antigen
Synonyms TSC2, fc16b02, tuberin, wu:fc16b02, LAM, TSC4, Nafld, Tcs2, Rc
Epitope
C-Term
(38), (32), (30), (27), (26), (25), (24), (24), (23), (22), (22), (21), (16), (16), (16), (16), (16), (16), (16), (16), (16), (16), (15), (14), (12), (12), (12), (12), (12), (12), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(519), (382), (240)
Host
Rabbit
(668), (26)
Clonality
Polyclonal
Conjugate
Un-conjugated
(55), (55), (55), (43), (43), (43), (12), (12), (12), (12), (12), (12), (12), (12)
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
(338), (257), (227), (149), (120), (66), (66), (36), (14), (8), (6), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen TSC2 antibody was raised against a 15 amino acid peptide from near the carboxy-terminus of human TSC2.
Isotype IgG
Specificity This antibody detects Tuberin / TSC2 at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Alternative Name Tuberin / TSC2 (TSC2 Antibody Abstract)
Background Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). At least three isoforms of TSC2 exist.Synonyms: TSC4, Tuberous sclerosis 2 protein
Gene ID 7249
NCBI Accession NP_000539
Application Notes ELISA. Western blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Background publications Inoki, Ouyang, Li et al.: "Signaling by target of rapamycin proteins in cell growth control." in: Microbiology and molecular biology reviews : MMBR, Vol. 69, Issue 1, pp. 79-100, 2005 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Inoki, Li, Xu et al.: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003 (PubMed).

Tabancay, Gau, Machado et al.: "Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39921-30, 2003 (PubMed).

Validation Images
Catalog No. ABIN501073
401.50 $
Plus shipping costs $45.00
Quantity
Price
0.1 mg
401.50 $

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