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UBE2V1 antibody (Ubiquitin-Conjugating Enzyme E2 Variant 1) (C-Term)

Details for Product anti-UBE2V1 Antibody No. ABIN501100, Supplier: Log in to see
Antigen
  • 0610011J09Rik
  • AI256840
  • CIR1
  • CROC-1
  • CROC-1B
  • CROC1
  • D7Bwg1382e
  • UBE2V
  • UEV-1
  • UEV1
  • UEV1A
Alternatives
anti-Human UBE2V1 antibody for ELISA
Epitope
C-Term
29
10
7
4
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
78
31
24
Host
Rabbit
63
12
5
Clonality
Polyclonal
Conjugate
This UBE2V1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
65
36
11
7
4
4
1
1
Supplier
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Immunogen UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C- terminus of human UEV1A.
Isotype IgG
Specificity This antibody detects UBE2V1 / CROC1.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Ion exchange chromatography
Alternative Name UBE2V1 / CROC1 (UBE2V1 Antibody Abstract)
Background Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (1). There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).Synonyms: CROC-1, P/OKcl.19, TRAF6-regulated IKK activator 1 beta Uev1A, UBE2V, UEV-1, UEV1, Ubiquitin-conjugating enzyme E2 variant 1
Gene ID 387522
NCBI Accession NP_954595
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes ELISA. Western blot: 1 to 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-UBE2V1 antibody (Ubiquitin-Conjugating Enzyme E2 Variant 1) (C-Term) (ABIN501100) Western blot analysis of UEV1A in Jurkat cell lysates with this product at (A) 1, (B)...
Background publications Akira, Takeda: "Toll-like receptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 7, pp. 499-511, 2004

Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004

Deng, Wang, Spencer, Yang, Braun, You, Slaughter, Pickart, Chen: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000

Sancho, Vilá, Sánchez-Pulido, Lozano, Paciucci, Nadal, Fox, Harvey, Bercovich, Loukili, Ciechanover, Lin, Sanz, Estivill, Valencia, Thomson: "Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 576-89, 1998