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XiAP Associated Factor 1 (XAF1) (Middle Region) antibody

Details for Product No. ABIN501128, Supplier: Login to see
Antigen
  • BIRC4BP
  • HSXIAPAF1
  • XIAPAF1
  • Fbxo39
Epitope
Middle Region
24
16
16
10
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
66
33
7
Host
Rabbit
80
5
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
78
28
19
16
4
4
2
1
Supplier
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Immunogen XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1.
Isotype IgG
Specificity This antibody detects XAF-1 at Center.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity chromatography
Alternative Name XAF1 (XAF1 Antibody Abstract)
Background XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-α2 and IFN-β can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).Synonyms: BIRC4-binding protein, BIRC4BP, XIAP-associated factor 1, XIAPAF1
Gene ID 54739
NCBI Accession NP_059993
Pathways
Application Notes ELISA. Western blot: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-XiAP Associated Factor 1 (XAF1) (Middle Region) antibody (ABIN501128) Western blot analysis of XAF-1 in human spleen lysate with AP30989PU-N (IN) at 1 (lan...
Background publications Liston, Fong, Kelly et al.: "Identification of XAF1 as an antagonist of XIAP anti-Caspase activity." in: Nature cell biology, Vol. 3, Issue 2, pp. 128-33, 2001 (PubMed).