XiAP Associated Factor 1 (XAF1) (Middle Region) antibody

Details for Product No. ABIN501128
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Antigen
Synonyms BIRC4BP, HSXIAPAF1, XIAPAF1, Fbxo39
Epitope
Middle Region
(14), (9), (8), (7), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(52), (36), (18)
Host
Rabbit
(70), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(59), (22), (19), (11), (10), (4), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN501128
401.50 $
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Immunogen XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1.
Isotype IgG
Specificity This antibody detects XAF-1 at Center.
Purification Affinity chromatography
Alternative Name XAF1
Background XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and –9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).
Alternate names: BIRC4-binding protein, BIRC4BP, XIAP-associated factor 1, XIAPAF1
Gene ID 54739
NCBI Accession NP_059993
Application Notes ELISA. Western blot: 1-2 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Liston, Fong, Kelly et al.: "Identification of XAF1 as an antagonist of XIAP anti-Caspase activity." in: Nature cell biology, Vol. 3, Issue 2, pp. 128-33, 2001 (PubMed).

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