CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (N-Term) antibody

Details for Product No. ABIN501510
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Antigen
Synonyms CTCFL, BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2, Boris, OTTMUSG00000016680
Epitope
N-Term
(12), (5), (3), (1), (1)
Reactivity
Human
(26), (19), (16), (12), (12), (12), (1)
Host
Rabbit
(29), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(22), (10), (5), (5), (3), (3), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN501510
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human CTCFL
Sequence CREKDHRSPS ELEAERTSGA FQDSVLEEEV ELVLAPSEES EKYILTLQTV
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against CTCFL. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CTCFL
Background CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. CTCFL is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and CTCFL are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation.CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation.
Molecular Weight 76 kDa
Gene ID 140690
NCBI Accession NP_542185, NM_080618
UniProt Q8NI51
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 663 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (N-Term) antibody WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate
Product cited in: Sun, Huang, Nguyen et al.: "DNA methyltransferase 1 and 3B activate BAG-1 expression via recruitment of CTCFL/BORIS and modulation of promoter histone methylation." in: Cancer research, Vol. 68, Issue 8, pp. 2726-35, 2008 (PubMed).

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