Topoisomerase (DNA) II beta 180kDa (TOP2B) (Middle Region) antibody

Details for Product No. ABIN501630
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Antigen
Synonyms TOP2B, D230016L12Rik, Top-2, TOPIIB, top2beta
Epitope
Middle Region
(8), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Human
(51), (18), (3)
Host
Rabbit
(51), (2)
Clonality
Polyclonal
Application
Western Blotting (WB)
(33), (25), (21), (16), (6), (4), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN501630
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Immunogen Synthetic peptide directed towards the middle region of human TOP2B
Sequence ENEGDYNPGR KTSKTTSKKP KKTSFDQDSD VDIFPSDFPT EPPSLPRTGR
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against TOP2B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name TOP2B
Background TOP2B is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants, however, the second variant has not yet been fully described.
Molecular Weight 183 kDa
Gene ID 7155
NCBI Accession NP_001059, NM_001068
UniProt Q02880
Research Area DNA/RNA, Cell Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1621 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (Middle Region) antibody WB Suggested Anti-TOP2B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate
anti-Topoisomerase (DNA) II beta 180kDa (TOP2B) (Middle Region) antibody (2) WB Suggested Anti-TOP2B
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate
TOP2B is supported by BioGPS gene expression data to be expressed in Jurkat
Background publications McNamara, Wang, Hanna et al.: "Topoisomerase IIbeta negatively modulates retinoic acid receptor alpha function: a novel mechanism of retinoic acid resistance." in: Molecular and cellular biology, Vol. 28, Issue 6, pp. 2066-77, 2008 (PubMed).

Validation Images
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