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Helicase-Like Transcription Factor (HLTF) (N-Term) antibody

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Antigen
Synonyms smarca3, wu:fc33f07, HLTF, SMARCA3, MGC131155, HIP116, HIP116A, HLTF1, RNF80, SNF2L3, ZBU1, RUSH, AF010600, BC057116, P113, Smarca3, Snf2l3
Epitope
N-Term
(18), (6), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit
(70), (28), (28), (8), (6), (6), (1), (1)
Host
Rabbit
(53), (17), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(54), (25), (11), (7), (4), (4), (3), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human HLTF
Blocking Peptide Blocking peptide for this product available: ABIN978033
Sequence SWMFKRDPVW KYLQTVQYGV HGNFPRLSYP TFFPRFEFQD VIPPDDFLTS
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 100 %, Mouse : 70 %
Characteristics This is a rabbit polyclonal antibody against HLTF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HLTF (HLTF Antibody Abstract)
Background SMARCA3 (HLTF) is a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA3 contains a RING finger DNA binding motif. This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.
Molecular Weight 114 kDa
Gene ID 6596
NCBI Accession NM_003071, NP_003062
UniProt Q14527
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1009 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Helicase-Like Transcription Factor (HLTF) (N-Term) antibody (ABIN501652) WB Suggested Anti-HLTF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Background publications Unk, Hajdú, Fátyol et al.: "Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 10, pp. 3768-73, 2008 (PubMed).

Catalog No. ABIN501652
$289.00
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