Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Middle Region) antibody

Details for Product No. ABIN501932
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Antigen
Synonyms
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, gl ... show more
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, glyceraldehyde-3-phosphate dehydrogenase C2, BEST:GH12586, CG12055, Dmel\\CG12055, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, KNC-NDS6, G3PD, cb609, mg:bb02e05, wu:fb33a10, wu:ft80f05, G3PDH show less
Epitope
Middle Region
(85), (38), (14), (13), (10), (8), (8), (7), (7), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Human
(400), (217), (194), (95), (37), (30), (28), (27), (17), (17), (16), (13), (12), (12), (9), (9), (8), (8), (7), (6), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(275), (191), (34), (18), (17)
Clonality
Polyclonal
Conjugate
Un-conjugated
(31), (28), (12), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(448), (226), (126), (78), (76), (74), (49), (31), (17), (10), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN501932
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human GAPDH
Sequence KAGAHLQGGA KRVIISAPSA DAPMFVMGVN HEKYDNSLKI ISNASCTTNC
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Dog : 90 %, Horse : 90 %, Mouse : 90 %, Pig : 90 %, Rabbit : 90 %, Rat : 90 %, Chicken : 80 %
Characteristics This is a rabbit polyclonal antibody against GAPDH. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name GAPDH
Background GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
Molecular Weight 36 kDa
Gene ID 2597
NCBI Accession NP_002037, NM_002046
UniProt P04406
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 335 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Background publications Rinne, Clements, Lamme et al.: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, 2008 (PubMed).

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