PIGZ antibody (N-Term)

Catalog No. ABIN502056

Product Details anti-PIGZ Antibody

Target: PIGZ (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Z (PIGZ))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGZ antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VLWGGLSLLR VLWCLLPQTG YVHPDEFFQS PEVMAEDILG VQAARPWEFY

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Yeast: 80%

Characteristics: This is a rabbit polyclonal antibody against PIGZ. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIGZ

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 579 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIGZ antibody (ABIN502056)

Taron, Colussi, Wiedman, Orlean, Taron: "Human Smp3p adds a fourth mannose to yeast and human glycosylphosphatidylinositol precursors in vivo." in: The Journal of biological chemistry, Vol. 279, Issue 34, pp. 36083-92, (2004) (PubMed).

Target Details for PIGZ

Target: PIGZ (Phosphatidylinositol Glycan Anchor Biosynthesis, Class Z (PIGZ))

Alternative Name: PIGZ (PIGZ Products)

Synonyms: F630022B06Rik antibody, GPI-MT-IV antibody, PIG-Z antibody, SMP3 antibody, phosphatidylinositol glycan anchor biosynthesis, class Z antibody, phosphatidylinositol glycan anchor biosynthesis class Z antibody, Pigz antibody, PIGZ antibody

Background: The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. PIGZ is a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.
Alias Symbols: FLJ12768, GPI-MT-IV, MGC52163, SMP3, PIG-Z
Protein Size: 579

Molecular Weight: 63 kDa

Gene ID: 80235

NCBI Accession: NM_025163, NP_079439

UniProt: Q86VD9

Pathways: Inositol Metabolic Process