Lamin B1 antibody (Middle Region)

Catalog No. ABIN502713

Product Details anti-Lamin B1 Antibody

Target: Lamin B1 (LMNB1)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EEVAQRSTVF KTTIPEEEEE EEEAAGVVVE EELFHQQGTP RASNRSCAIM

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 91%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against LMNB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LMNB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 586 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Lamin B1 antibody (ABIN502713)

Lee, Seng, Sekine, Hinton, Fu, Avraham, Avraham: "Vascular endothelial growth factor mediates intracrine survival in human breast carcinoma cells through internally expressed VEGFR1/FLT1." in: PLoS medicine, Vol. 4, Issue 6, pp. e186, (2007) (PubMed).

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: LMNB1 (LMNB1 Products)

Synonyms: MGC52550 antibody, Lamin-L(I) antibody, lamin-b antibody, lmn antibody, lmn2 antibody, lmnb antibody, LMNB1 antibody, ADLD antibody, LMN antibody, LMN2 antibody, LMNB antibody, fc06g01 antibody, wu:fc06g01 antibody, lamin B1 L homeolog antibody, lamin B1 antibody, lmnb1.L antibody, lmnb1 antibody, LMNB1 antibody, Lmnb1 antibody

Background: LAMNB1 encodes for one of the two B type proteins of the nuclear lamina, B1. The Vertebrate lamins consist of two types, A and B. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression.The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ADLD, LMN, LMN2, LMNB, MGC111419
Protein Interaction Partner: POU2F1, SPRTN, UBC, SUMO2, SUMO3, MDM2, EED, ACTR10, NCAPH, ZZEF1, DCTN3, DCTN2, ACTR1A, ACTR1B, PARK2, HECW2, YWHAQ, ADRB2, LMNA, MELK, URB2, FN1, ATF4, SERPINA3, ABCG8, MBIP, GIT2, WHSC1, VCAM1, ITGA4, PRKCB, CDK1, MAGT1, HP1BP3, NCSTN, HNRNPM, EIF6, IL
Protein Size: 586

Molecular Weight: 66 kDa

Gene ID: 4001

NCBI Accession: NM_005573, NP_005564

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis