MELK antibody (Middle Region)

Catalog No. ABIN502726

Product Details anti-MELK Antibody

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AVKNEEYFMF PEPKTPVNKN QHKREILTTP NRYTTPSKAR NQCLKETPIK

Predicted Reactivity: Cow: 79%, Dog: 93%, Guinea Pig: 80%, Horse: 100%, Human: 100%, Rabbit: 87%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MELK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MELK

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 651 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK (MELK Products)

Synonyms: MELK antibody, xmelk antibody, Melk antibody, HPK38 antibody, AI327312 antibody, MPK38 antibody, mKIAA0175 antibody, fb71b01 antibody, fe01f04 antibody, wu:fb71b01 antibody, wu:fe01f04 antibody, maternal embryonic leucine zipper kinase antibody, maternal embryonic leucine zipper kinase L homeolog antibody, MELK antibody, melk antibody, cgd5_2270 antibody, Melk antibody, melk.L antibody

Background: The protein MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. Also it may be involved in the inhibition of spliceosome assembly during mitosis.
Alias Symbols: HPK38, KIAA0175
Protein Interaction Partner: UBC, YGR054W, Acaca, CSN1S1, MELK, MBP, LMNB1, BABAM1, ZNF622, SF3B1, EIF2S1, CDC5L, PPP1R8, HIST1H1B, CDC25B,
Protein Size: 651

Molecular Weight: 75 kDa

Gene ID: 9833

NCBI Accession: NM_014791, NP_055606

UniProt: Q14680