Nicotinamide Nucleotide Transhydrogenase (NNT) (N-Term) antibody

Details for Product No. ABIN502788
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Antigen
Synonyms Afu5g02780, GCCD4, 4930423F13Rik, AI323702, BB168308, wu:fa20d10, wu:fc86a04, zgc:76979
Epitope
N-Term
(14), (7), (3), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Xenopus laevis, Cow (Bovine), Zebrafish (Danio rerio), Human, Chicken
(30), (16), (16), (12), (12), (12), (12)
Host
Rabbit
(21), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(21), (10), (10), (7), (5), (4), (3), (2)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN502788
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Immunogen Synthetic peptide directed towards the N terminal of human NNT
Sequence IVRGFDTRAA ALEQFKSLGA EPLEVDLKES GEGQGGYAKE MSKEFIEAEM
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Chicken : 90 %, Zebrafish : 90 %  
Characteristics This is a rabbit polyclonal antibody against NNT. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NNT
Background NNT is an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.
Molecular Weight 114 kDa
Gene ID 23530
NCBI Accession NP_036475, NM_012343
UniProt Q13423
Research Area Signaling, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1086 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (N-Term) antibody WB Suggested Anti-NNT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Liver
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (N-Term) antibody (2) Host: Rabbit
Target Name: NNT
Sample Tissue: Hela Whole cell
Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5 µg/mL Lysate Quantity: 41 µg/lane/LaneGel Concentration:.12
Product cited in: Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

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