B3GALTL antibody (Middle Region)

Catalog No. ABIN503023

Product Details anti-B3GALTL Antibody

Target: B3GALTL (beta 1,3-Galactosyltransferase-Like (B3GALTL))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Dog, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B3GALTL antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DYPKDYLSHQ VPISFHKHWN IDPVKVYFTW LAPSDEDKAR QETQKGFREE

Predicted Reactivity: Dog: 93%, Human: 100%, Mouse: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against B3GALTL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human B3GALTL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 498 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for B3GALTL

Target: B3GALTL (beta 1,3-Galactosyltransferase-Like (B3GALTL))

Alternative Name: B3GALTL (B3GALTL Products)

Synonyms: B3GLCT antibody, B3GTL antibody, B3Glc-T antibody, Gal-T antibody, beta3Glc-T antibody, Gm1057 antibody, beta 3-glucosyltransferase antibody, beta 3-glucosyltransferase L homeolog antibody, beta-3-glucosyltransferase antibody, B3GLCT antibody, b3glct antibody, b3glct.L antibody, B3glct antibody

Background: B3GALTL is the O-fucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. B3GALTL specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.B3GALTL is a beta-1,3-glucosyltransferase involved in the synthesis of the unusual O-linked disaccharide glucosyl-beta-1,3-fucose-O- found on the thrombospondin (see THBS1, MIM 188060) type-1 repeats (TSRs) of many biologically important proteins. Biosynthesis of glucosyl-beta-1,3-fucose-O- is initiated by protein O-fucosyltransferase-2 (POFUT2, MIM 610249), which attaches the fucosyl residue to a serine or threonine within the TSR. B3GALTL subsequently transfers the glucose onto TSR-fucose (Hess et al., 2008 [PubMed 18199743]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-270 DN999521.1 41-310 271-457 AB101481.1 161-347 458-467 DN999521.1 499-508 468-1217 AB101481.1 358-1107 1218-1279 DA290408.1 515-576 1280-1607 AB101481.1 1170-1497 1608-2495 AY190526.1 1498-2385 2496-3459 AY190526.1 2387-3350 3460-4071 AV728071.1 87-698 4072-4221 AA769548.1 1-150 c
Alias Symbols: B3GTL, B3Glc-T, Gal-T, beta3Glc-T, B3GLCT
Protein Interaction Partner: UBC, ELAVL1,
Protein Size: 498

Molecular Weight: 56 kDa

Gene ID: 145173

NCBI Accession: NM_194318, NP_919299

UniProt: Q6Y288