Homeodomain Interacting Protein Kinase 4 (HIPK4) (Middle Region) antibody

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Antigen
Synonyms Gm162
Epitope
Middle Region
(20), (15), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(71), (40), (24), (12)
Host
Rabbit
(72), (15)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(71), (45), (40), (13), (11), (9), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human HIPK4
Blocking Peptide Blocking peptide for this product available: Homeodomain Interacting Protein Kinase 4 (HIPK4) (Middle Region) peptide (ABIN977996)
Sequence AEEKEAAGMG SVAGSSPFFR EEKAPGMQRA IDQLDDLSLQ EAGHGLWGET
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against HIPK4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HIPK4 (HIPK4 Antibody Abstract)
Background HIPK4 is a protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter. It may act as a corepressor of transcription factors.
Molecular Weight 69 kDa
Gene ID 147746
NCBI Accession NM_144685, NP_653286
UniProt Q8NE63
Research Area Chromatin and Nuclear Signaling, Cell Cycle, Transcription Factors, Apoptosis/Necrosis
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 616 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Homeodomain Interacting Protein Kinase 4 (HIPK4) (Middle Region) antibody (ABIN503376) WB Suggested Anti-HIPK4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Arai, Matsushita, Du et al.: "Novel homeodomain-interacting protein kinase family member, HIPK4, phosphorylates human p53 at serine 9." in: FEBS letters, Vol. 581, Issue 29, pp. 5649-57, 2007 (PubMed).

Catalog No. ABIN503376
$289.00
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