Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (Middle Region) antibody

Details for Product No. ABIN503488
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Antigen
Synonyms fa16e07, wu:fa16c06, wu:fa16e07, wu:fc18c08, zgc:55659, ADTB2, AP105B, AP2-BETA, CLAPB1, 1300012O03Rik, AI788979
Epitope
Middle Region
(8), (7), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Dog (Canine), Cow (Bovine), Human, Chicken
(45), (22), (20), (14), (14), (12), (2), (2), (2)
Host
Rabbit
(43), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(36), (16), (10), (8), (6), (2)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN503488
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human AP2B1
Sequence DMLYQSLKLT NGIWILAELR IQPGNPNYTL SLKCRAPEVS QYIYQVYDSI
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against AP2B1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name AP2B1
Background AP2B1 is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. AP2B1 is found on the cytoplasmic face of coated vesicles in the plasma membrane.The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 105 kDa
Gene ID 163
NCBI Accession NM_001030006, NP_001025177
Research Area Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 951 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (Middle Region) antibody WB Suggested Anti-AP2B1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: OVCAR-3 cell lysate
Product cited in: Schmid, Ford, Burtey et al.: "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly." in: PLoS biology, Vol. 4, Issue 9, pp. e262, 2006 (PubMed).

Validation Images
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