Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (Middle Region) antibody

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Antigen
Synonyms fa16e07, wu:fa16c06, wu:fa16e07, wu:fc18c08, zgc:55659, ADTB2, AP105B, AP2-BETA, CLAPB1, 1300012O03Rik, AI788979
Alternatives
Epitope
Middle Region
(19), (15), (10), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(65), (25), (20), (4), (4), (2), (2), (2), (2)
Host
Rabbit
(60), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1)
Application
Western Blotting (WB)
(59), (31), (17), (4), (3), (2), (2), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human AP2B1
Blocking Peptide Blocking peptide for this product available: Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (Middle Region) peptide (ABIN973280)
Sequence DMLYQSLKLT NGIWILAELR IQPGNPNYTL SLKCRAPEVS QYIYQVYDSI
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against AP2B1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name AP2B1 (AP2B1 Antibody Abstract)
Background AP2B1 is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. AP2B1 is found on the cytoplasmic face of coated vesicles in the plasma membrane.The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 105 kDa
Gene ID 163
NCBI Accession NM_001030006, NP_001025177
Research Area Signaling
Pathways EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 951 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1) (Middle Region) antibody (ABIN503488) WB Suggested Anti-AP2B1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Product cited in: Schmid, Ford, Burtey et al.: "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly." in: PLoS biology, Vol. 4, Issue 9, pp. e262, 2006 (PubMed).

Catalog No. ABIN503488
$289.00
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