Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Middle Region) antibody

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Antigen
Synonyms APPL, DIP13alpha, 2900057D21Rik, 7330406P05Rik, AI585782, AW209077, BB022931, C88264, DIP13, RGD1309388
Epitope
Middle Region
(22), (9), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(58), (26), (16), (4), (4), (1), (1), (1), (1), (1)
Host
Rabbit
(43), (16), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(59), (36), (13), (9), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human APPL1
Blocking Peptide Blocking peptide for this product available: Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Middle Region) peptide (ABIN973345)
Sequence GQAKAFGQGG RRTNPFGESG GSTKSETEDS ILHQLFIVRF LGSMEVKSDD
Predicted Reactivity Guinea pig : 100 %, Rat : 100 %, Dog : 90 %, Horse : 90 %, Bovine : 80 %, Chicken : 80 %, Pig : 80 %
Characteristics This is a rabbit polyclonal antibody against APPL1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name APPL1 (APPL1 Antibody Abstract)
Background The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A
Molecular Weight 80 kDa
Gene ID 26060
NCBI Accession NM_012096, NP_036228
UniProt Q9UKG1
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 709 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Middle Region) antibody (ABIN503582) WB Suggested Anti-APPL1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications McCrea, Paradise, Tomasini et al.: "All known patient mutations in the ASH-RhoGAP domains of OCRL affect targeting and APPL1 binding." in: Biochemical and biophysical research communications, Vol. 369, Issue 2, pp. 493-9, 2008 (PubMed).

Catalog No. ABIN503582
$289.00
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