MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody

Antigen: MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1)

Synonyms: FCC2, COCA2, HNPCC, hMLH1, HNPCC2, MGC5172, MGC66301, zgc:66301, MLH1, MGC140127, LOC100232198

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Pig (Porcine), Fruit Fly (Drosophila melanogaster), Chicken

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN503836

Additional Information

Alternative name: MLH1

Gene ID: MLH1

UniProt: P40692

Immunogen: The immunogen for anti-MLH1 antibody: synthetic peptide directed towards the N terminal of human MLH1

Sequence: MIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKEDLD IVCERFTTSK

Cross-Reactivity: Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-MLH1 antibody concentration is 1 mg/ml.

Description: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC.This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Characteristics: Antigen Size: 756 amino acids

Molecular Weight: 84kDa

Application Details

Application Notes: WB Suggested Anti-MLH1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Research Area: Cancer, DNA/RNA

Restrictions: For Research Use only

Images

WB Suggested Anti-MLH1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta

Publications

Product: Harley, Rosen, Risch et al.: "Ovarian cancer risk is associated with a common variant in the promoter sequence of the mismatch repair gene MLH1." in: Gynecologic oncology, Vol. 109, Issue 3, pp. 384-7, 2008 (PubMed).