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Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (Middle Region) antibody

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Antigen
Synonyms cb1034, mmp13, MMP13, xCol, xcl3, MMP-13, MGC108008, Clg, Mmp1, CLG3, MANDP1, gene A
Epitope
Middle Region
(31), (24), (15), (12), (11), (10), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis
(209), (52), (50), (14), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(201), (67), (10), (3), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(26), (18), (11), (4), (4), (4), (1), (1)
Application
Western Blotting (WB)
(202), (108), (95), (67), (53), (41), (20), (19), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human MMP13
Blocking Peptide Blocking peptide for this product available: ABIN980149
Sequence HFMLPDDDVQ GIQSLYGPGD EDPNPKHPKT PDKCDPSLSL DAITSLRGET
Predicted Reactivity Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Rabbit : 100 %, Dog : 90 %, Mouse : 90 %, Rat : 90 %, Sheep : 90 %, Chicken : 90 %, Bovine : 80 %, African clawed frog : 80 %
Characteristics This is a rabbit polyclonal antibody against MMP13. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MMP13 (MMP13 Antibody Abstract)
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as art
Molecular Weight 42 kDa
Gene ID 4322
NCBI Accession NP_002418, NM_002427
UniProt P45452
Research Area Cancer, Proteolysis / Ubiquitin, Metalloprotease, Angiogenesis, Extracellular Matrix, Matrix Metalloproteinases
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 471 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (Middle Region) antibody (ABIN503937) WB Suggested Anti-MMP13 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (Middle Region) antibody (ABIN503937) Sample Type: Equine Cartilage Explants
Primary Dilution: 1:1000
Western Blotting (WB) image for anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (Middle Region) antibody (ABIN503937) Sample Type: 1. Normal HUman Cartilage (50ug)
2. Human Osteoarthritis carti...
Product cited in: Park, David, Park et al.: "Controlled release of simvastatin from in situ forming hydrogel triggers bone formation in MC3T3-E1 cells." in: The AAPS journal, Vol. 15, Issue 2, pp. 367-76, 2013 (PubMed).

Background publications Borghese, Chiche, Vernerey et al.: "Genetic polymorphisms of matrix metalloproteinase 12 and 13 genes are implicated in endometriosis progression." in: Human reproduction (Oxford, England), Vol. 23, Issue 5, pp. 1207-13, 2008 (PubMed).

Catalog No. ABIN503937
$289.00
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