RAB5A, Member RAS Oncogene Family (RAB5A) (Middle Region) antibody

Details for Product No. ABIN504007
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Antigen
Synonyms DDBDRAFT_0168594, DDBDRAFT_0229401, DDB_0168594, DDB_0229401, rab5, RAB5A, RAB5, 2410015H04Rik, AI663973, AU021172, nnyRab5a
Epitope
Middle Region
(15), (13), (10), (6), (6), (6), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Human
(110), (61), (46), (22), (18), (14), (9), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Host
Rabbit
(92), (24), (4), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(107), (27), (25), (22), (11), (10), (10), (10), (7), (3), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
Options
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Catalog No. ABIN504007
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human RAB5A
Sequence KTSMNVNEIF MAIAKKLPKN EPQNPGANSA RGRGVDLTEP TQPTRNQCCS
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Bovine : 90 %, Chicken : 80 %, Pig : 70 %, Sheep : 70 %
Characteristics This is a rabbit polyclonal antibody against RAB5A. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RAB5A
Background RAB5A is required for the fusion of plasma membranes and early endosomes.
Molecular Weight 24 kDa
Gene ID 5868
NCBI Accession NM_004162, NP_004153
UniProt P20339
Research Area Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 215 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (Middle Region) antibody WB Suggested Anti-RAB5A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Muscle
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (Middle Region) antibody (2) Sample type: zebra gut epithelial cells
Green: primary
Red: actin
Blue: DAPI
Primary dilution: 1:5000
Secondary Dilution: 1:300
Image Submitted by: Michel Bagnat
Duke University Medical Center .

anti-RAB5A, Member RAS Oncogene Family (RAB5A) (Middle Region) antibody (3) Sample type: zebra gut epithelial cells
Green: primary
Red: actin
Blue: DAPI
Primary dilution: 1:5000
Secondary Dilution: 1:300
Image Submitted by: Michel Bagnat
Duke University Medical Center .

Background publications Coyne, Shen, Turner et al.: "Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5." in: Cell host & microbe, Vol. 2, Issue 3, pp. 181-92, 2007 (PubMed).

Validation Images
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