Did you know that you can buy products from over 140 different suppliers from us?

Centromere Protein H (CENPH) (Middle Region) antibody

Details for Product No. ABIN504443
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ENP, CENP-H, AU044255, 1700021I11Rik, 2410018A12Rik, 2610042E16Rik, 2810046K12Rik, si:dkey-186m8.3, cenp-h
Epitope
Middle Region
(7), (6), (2), (1)
Reactivity
Mouse (Murine), Cow (Bovine), Dog (Canine), Human
(47), (18), (16), (12), (12)
Host
Rabbit
(33), (16)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(38), (15), (14), (10), (7), (6), (5), (4), (3), (1)
Pubmed 2 references available
Quantity 50 µg
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN504443
289.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide directed towards the middle region of human CENPH
Sequence ESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKID LDSMENSERI
Predicted Reactivity Horse : 100 %, Human : 100 %, Bovine : 90 %, Mouse : 90 %, Dog : 70 %
Characteristics This is a rabbit polyclonal antibody against CENPH. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CENPH
Background Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
Molecular Weight 27 kDa
Gene ID 64946
NCBI Accession NM_022909, NP_075060
UniProt Q9H3R5
Research Area Cancer, Cell Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 247 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Centromere Protein H (CENPH) (Middle Region) antibody WB Suggested Anti-CENPH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Liver
Product cited in: Liao, Zhang, Zhou et al.: "Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity." in: Cancer research, Vol. 67, Issue 17, pp. 8131-8, 2007 (PubMed).

Background publications Liao, Song, Zhang et al.: "Centromere protein H is a novel prognostic marker for nasopharyngeal carcinoma progression and overall patient survival." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 2 Pt 1, pp. 508-14, 2007 (PubMed).

Hosts (33), (16)
Reactivities (47), (18), (16), (12), (12)
Applications (38), (15), (14), (10), (7), (6), (5), (4), (3), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (6), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN504443
289.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top