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Transcription Factor 12 (TCF12) (Middle Region) antibody

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Antigen
Synonyms TCF12, CRS3, HEB, HTF4, HsT17266, bHLHb20, A130037E08Rik, ALF1, HEBAlt, HTF-4, ME1, REB, SCBPA, wu:fb74g05, wu:fc43e06, zgc:110341, zgc:85956
Epitope
Middle Region
(32), (16), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(75), (29), (29), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(67), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(60), (33), (13), (12), (11), (4), (4), (3), (2), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the middle region of human TCF12
Sequence GGLQSQSGTV VTTEIKTENK EKDENLHEPP SSDDMKSDDE SSQKDIKVSS
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %
Purification Affinity Purified
Alternative Name TCF12 (TCF12 Antibody Abstract)
Background TCF12 encodes a protein that is a member of the basic helix-loop-helix (bHLH) E-protein family which recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cell. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Molecular Weight 73 kDa
NCBI Accession NM_003205, NP_003196
UniProt Q99081
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes WB Suggested Anti-TCF12 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 682 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Any unfrozen and/or unused material can be stored at 4 °C for short term use (< 1 week), and should not be re-frozen. For longer periods of storage, store at -24 °C
Supplier Images
Western Blotting (WB) image for anti-Transcription Factor 12 (TCF12) (Middle Region) antibody (ABIN504648) WB Suggested Anti-TCF12 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Wissmüller, Kosian, Wolf et al.: "The high-mobility-group domain of Sox proteins interacts with DNA-binding domains of many transcription factors." in: Nucleic acids research, Vol. 34, Issue 6, pp. 1735-44, 2006 (PubMed).

Catalog No. ABIN504648
$289.00
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