KIR2DL4/CD158d antibody

Catalog No. ABIN509568

Product Details anti-KIR2DL4/CD158d Antibody

Target: KIR2DL4/CD158d (KIR2DL4) (Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 4 (KIR2DL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIR2DL4/CD158d antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d / KIR2DL4, a 45 kDa NK cell marker. Cell surface expression and function of CD158d / KIR2DL4 depends on genotype of particular individuals.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: NK3.3 cells and KIR2DL4-Ig fusion protein

Clone: MAb 33

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-6 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-KIR2DL4/CD158d antibody (ABIN509568)

Rajagopalan, Bryceson, Kuppusamy, Geraghty, van der Meer, Joosten, Long: "Activation of NK cells by an endocytosed receptor for soluble HLA-G." in: PLoS biology, Vol. 4, Issue 1, pp. e9, (2006) (PubMed).

LeMaoult, Zafaranloo, Le Danff, Carosella: "HLA-G up-regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 6, pp. 662-4, (2005) (PubMed).

Yan, Fan: "Residues Met76 and Gln79 in HLA-G alpha1 domain involve in KIR2DL4 recognition." in: Cell research, Vol. 15, Issue 3, pp. 176-82, (2005) (PubMed).

Goodridge, Witt, Christiansen, Warren: "KIR2DL4 (CD158d) genotype influences expression and function in NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 4, pp. 1768-74, (2003) (PubMed).

Rajagopalan, Fu, Long: "Cutting edge: induction of IFN-gamma production but not cytotoxicity by the killer cell Ig-like receptor KIR2DL4 (CD158d) in resting NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 4, pp. 1877-81, (2001) (PubMed).

Target Details for KIR2DL4/CD158d

Target: KIR2DL4/CD158d (KIR2DL4) (Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 4 (KIR2DL4))

Alternative Name: CD158d / KIR2DL4 (KIR2DL4 Products)

Synonyms: G9P antibody, CD158D antibody, KIR103 antibody, KIR103AS antibody, KIR2DL4 antibody, killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4 antibody, killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4 antibody, KIR2DL4 antibody

Background: Killer cell immunoglobulin like receptor, two Ig d,CD158d / KIR2DL4 is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ, secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.,KIR2DL4, KIR103AS, 103AS, 15.212

Gene ID: 3805

UniProt: Q99706