NT5C2 antibody (AA 1-561)

Catalog No. ABIN524900

Product Details anti-NT5C2 Antibody

Target: NT5C2 (5'-Nucleotidase, Cytosolic II (NT5C2))

Binding Specificity: AA 1-561

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NT5C2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purpose: Mouse monoclonal antibody raised against a full length recombinant NT5C2.

Sequence: MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 3C1

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-NT5C2 antibody (ABIN524900)

Cividini, Tozzi, Galli, Pesi, Camici, Dumontet, Jordheim, Allegrini: "Cytosolic 5'-nucleotidase II interacts with the leucin rich repeat of NLR family member Ipaf." in: PLoS ONE, Vol. 10, Issue 3, pp. e0121525, (2015) (PubMed).

Jordheim, Puy, Cros-Perrial, Peyrottes, Lefebvre, Périgaud, Dumontet: "Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models." in: Analytical and bioanalytical chemistry, Vol. 407, Issue 19, pp. 5747-58, (2015) (PubMed).

Cividini, Cros-Perrial, Pesi, Machon, Allegrini, Camici, Dumontet, Jordheim, Tozzi: "Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II." in: The international journal of biochemistry & cell biology, Vol. 65, pp. 222-9, (2015) (PubMed).

Kulkarni, Karlsson, Szekeres, Chibalin, Krook, Zierath: "Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle." in: The Journal of biological chemistry, Vol. 286, Issue 40, pp. 34567-74, (2011) (PubMed).

Target Details for NT5C2

Target: NT5C2 (5'-Nucleotidase, Cytosolic II (NT5C2))

Alternative Name: NT5C2 (NT5C2 Products)

Synonyms: nt5c2 antibody, wu:fc36f11 antibody, wu:fc45b10 antibody, GMP antibody, NT5B antibody, PNT5 antibody, cN-II antibody, NT5C2 antibody, 2010002I23Rik antibody, CnII antibody, ENSMUSG00000025039 antibody, Gm9751 antibody, Gmp antibody, Nt5b antibody, Pnt5 antibody, im:7162273 antibody, zgc:153651 antibody, 5'-nucleotidase, cytosolic II antibody, 5'-nucleotidase, cytosolic IIa antibody, 5'-nucleotidase, cytosolic IIb antibody, NT5C2 antibody, nt5c2.L antibody, nt5c2 antibody, nt5c2a antibody, Nt5c2 antibody, surE2 antibody, nt5c2b antibody

Background: Full Gene Name: 5'-nucleotidase, cytosolic II
Synonyms: GMP,NT5B,PNT5,cN-II

Gene ID: 22978