Mouse monoclonal antibody raised against native NCAM1.
Specificity
This antibody reacts with a 180 KDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that This antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
Cross-Reactivity
Human, Primate
Immunogen
Native purified NCAM1 from KG-1 human acute myelogenous leukemia cell line.
NCAM1
Reactivity: Human, Rat, Chicken
FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 106 cells in a suspension. The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.2 % BSA, 0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
McCluggage, McKenna, McBride: "CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 26, Issue 3, pp. 322-7, (2007) (PubMed).
Ohishi, Kaku, Oya, Kobayashi, Wake, Tsuneyoshi: "CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls." in: Gynecologic oncology, Vol. 107, Issue 1, pp. 30-8, (2007) (PubMed).
Jakovcevski, Mo, Zecevic: "Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain." in: Neuroscience, Vol. 149, Issue 2, pp. 328-37, (2007) (PubMed).