Western Blot (1:1000) Immunohistochemistry (1:10-50) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).
McClanahan, Koseoglu, Smith, Grein, Gustafson, Black, Kirschmeier, Samatar: "Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors." in: Cancer biology & therapy, Vol. 5, Issue 4, pp. 419-26, (2006) (PubMed).
Yamamoto, Sakamoto, Fujii, Tsuiji, Kenetaka, Asaka, Hirohashi: "Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations." in: Hepatology (Baltimore, Md.), Vol. 37, Issue 3, pp. 528-33, (2003) (PubMed).
Target
LGR5
(Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))