SPAM1 antibody (Middle Region)

Catalog No. ABIN5517624

Product Details anti-SPAM1 Antibody

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAM1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QQQNVQLSLT EATEKAKQEF EKAGKDFLVE TIKLGKLLRP NHLWGYYLFP

Characteristics: This is a rabbit polyclonal antibody against SPAM1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human SPAM1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPAM1

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Alternative Name: SPAM1 (SPAM1 Products)

Synonyms: AV039194 antibody, Ph-20 antibody, PH-20 antibody, PH20 antibody, HYA1 antibody, HYAL1 antibody, HYAL3 antibody, HYAL5 antibody, SPAG15 antibody, Spam antibody, XEH1 antibody, spam1 antibody, im:6902904 antibody, zgc:158668 antibody, sperm adhesion molecule 1 antibody, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) L homeolog antibody, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) antibody, Spam1 antibody, SPAM1 antibody, spam1.L antibody, spam1 antibody

Background: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1, another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.

Alias Symbols: SPAM1, HYAL3, PH20,

Protein Size: 509

Gene ID: 6677

NCBI Accession: NP_694859

UniProt: P38567