COL1A1 antibody (C-Term)

Catalog No. ABIN5518708

Product Details anti-COL1A1 Antibody

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Binding Specificity: AA 1192-1207, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: QPPQEKSQDG GRYYRA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: collagen, type I, alpha 1
Protein Name: Collagen alpha-1(I) chain

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I(1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for COL1A1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-COL1A1 antibody (ABIN5518708)

Tang, Pei, Yang, Wang, Wang, Gao, Li, Yang, Yang: "The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-β1 pathway." in: Scientific reports, Vol. 6, pp. 29975, (2018) (PubMed).

Yao, Sun, Fei, Wang, Wang, Zhang, Wu, Liu, Liu, Cui, Li, Yu, Dang, Wang: "Screening in larval zebrafish reveals tissue-specific distribution of fifteen fluorescent compounds." in: Disease models & mechanisms, Vol. 10, Issue 9, pp. 1155-1164, (2018) (PubMed).

Shang, Liu, Liu, Li, Dou, Zhang, Sun, Zhang, Zhu, Mu, Ding, Wen: "Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-β1." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 4537-4544, (2018) (PubMed).

Zhou, Zhang, Gao, Tan, Zheng, Zhao, Zhang: "Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 473-484, (2018) (PubMed).

Yang, Jia, Liu, Ma: "Effect of genistein on myocardial fibrosis in diabetic rats and its mechanism." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 2929-2936, (2018) (PubMed).

"Retraction: Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192465, (2018) (PubMed).

Jiang, Zhong, Zheng, Zhao: "Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 2150-2158, (2018) (PubMed).

Zhang, Ju, Nie, Song, Xu, Gao: "Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells." in: International journal of molecular medicine, Vol. 42, Issue 1, pp. 279-289, (2018) (PubMed).

Zhao, Wang, Wang, Hong, Miao, Liao, Zhou, Liu: "An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease." in: Scientific reports, Vol. 8, Issue 1, pp. 8996, (2018) (PubMed).

Chen, Kong, Liu, Gao, Zhang, Li, Liu: "In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 95-104, (2017) (PubMed).

Zhao, Peng, Cui, Guo, Yan: "Dimethyl α-ketoglutarate reduces CCl4-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells." in: Biochemical and biophysical research communications, Vol. 481, Issue 1-2, pp. 90-96, (2017) (PubMed).

Huang, Jin, Lin, Lin, Zheng, Zhao: "Beneficial effects of sulfonamide‑based gallates on osteoblasts in vitro." in: Molecular medicine reports, Vol. 15, Issue 3, pp. 1149-1156, (2017) (PubMed).

et al.: "Erratum: Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway ..." in: International journal of nanomedicine, Vol. 12, pp. 1515, (2017) (PubMed).

Cao, Shi, Ge: "miR-21 enhances cardiac fibrotic remodeling and fibroblast proliferation via CADM1/STAT3 pathway." in: BMC cardiovascular disorders, Vol. 17, Issue 1, pp. 88, (2017) (PubMed).

Huang, Wu, Wang, Zheng, Zhao: "Protective effects of baicalin on rabbit articular chondrocytes in vitro." in: Experimental and therapeutic medicine, Vol. 13, Issue 4, pp. 1267-1274, (2017) (PubMed).

Cheng, Ge, Lan, Ma, Chi, Kuang, Sun, Zhao, Liu, Feng, Huang, Luo, Li, Zhang, Hu, Xu, Liu, Huo, Deng, Yang, Xi, Zhang, Siegenthaler, Chen: "Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity." in: Archives of toxicology, Vol. 92, Issue 1, pp. 469-485, (2017) (PubMed).

Wang, Shi, Xu, Qin, Wang: "Hesperetin alleviates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3713-3719, (2017) (PubMed).

Cui, Tang, Li, Wang, Rao, Wang, Liu, Chen: "Lipoxin A4 improves erectile dysfunction in rats with type I diabetes by inhibiting oxidative stress and corporal fibrosis." in: Asian journal of andrology, Vol. 20, Issue 2, pp. 166-172, (2017) (PubMed).

Wang, Yao, Shen, Ding, Shi, Chen, He, Ding, Zhang, Xie: "FOXA2 alleviates CCl4-induced liver fibrosis by protecting hepatocytes in mice." in: Scientific reports, Vol. 7, Issue 1, pp. 15532, (2017) (PubMed).

Zhang, Jin, Tian, Wu: "hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC." in: Oncotarget, Vol. 8, Issue 61, pp. 104508-104524, (2017) (PubMed).

Target Details for COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Alternative Name: COL1A1 (COL1A1 Products)

Synonyms: OI4 antibody, Col1a-1 antibody, Cola-1 antibody, Cola1 antibody, Mov-13 antibody, Mov13 antibody, COLIA1 antibody, col1a1 antibody, MGC52532 antibody, COL1A1 antibody, COL1A2 antibody, alpha1(I) antibody, cb21 antibody, sb:cb21 antibody, sb:cb384 antibody, wu:fa99c12 antibody, wu:fb02c06 antibody, alpha3(I) antibody, col1a3 antibody, hm:zeh0348 antibody, wu:fa95h05 antibody, wu:fd02a10 antibody, wu:fj59a10 antibody, collagen type I alpha 1 chain antibody, collagen, type I, alpha 1 antibody, collagen, type I, alpha 1 S homeolog antibody, collagen alpha-1(I) chain antibody, collagen type I alpha 2 chain antibody, collagen, type I, alpha 1a antibody, collagen 1a1 antibody, collagen, type I, alpha 1b antibody, COL1A1 antibody, Col1a1 antibody, col1a1.S antibody, LOC397571 antibody, COL1A2 antibody, col1a1a antibody, col1a1 antibody, col1a1b antibody

Background: Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Synonyms: Alpha 1 type I collagen antibody|Alpha 2 type I collagen antibody|Alpha-1 type I collagen antibody|CO1A1_HUMAN antibody|COL1A1 antibody|COL1A2 antibody|Collagen alpha-1(I) chain antibody|Collagen I alpha 1 polypeptide antibody|Collagen I alpha 2 polypeptide antibody|Collagen Of Skin Tendon And Bone antibody|Collagen Type 1 antibody|Collagen type I alpha 1 antibody|Collagen type I alpha 2 antibody|OI4 antibody|Osteogenesis Imperfecta Type IV antibody|Pro alpha 1(I) collagen antibody|Type I procollagen antibody

UniProt: P11087

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding