CHD2 antibody (AA 1124-1351)

Catalog No. ABIN5518901

Product Details anti-CHD2 Antibody

Target: CHD2 (Chromodomain Helicase DNA Binding Protein 2 (CHD2))

Binding Specificity: AA 1124-1351

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Chromodomain-helicase-DNA-binding protein 2(CHD2) detection. Tested with WB, IHC-P in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Chromodomain-helicase-DNA-binding protein 2(CHD2) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: chromodomain helicase DNA binding protein 2
Protein Name: Chromodomain-helicase-DNA-binding protein 2

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CHD2 recombinant protein (Position: R1124-K1351). Human CHD2 shares 97.4% amino acid (aa) sequence identity with mouse CHD2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CHD2

Target: CHD2 (Chromodomain Helicase DNA Binding Protein 2 (CHD2))

Alternative Name: CHD2 (CHD2 Products)

Synonyms: CHD2 antibody, MGC148727 antibody, MGC159952 antibody, DKFZp469E1718 antibody, EEOC antibody, 2810013C04Rik antibody, 2810040A01Rik antibody, 5630401D06Rik antibody, AI851092 antibody, BC029703 antibody, microRNA 3175 antibody, chromodomain helicase DNA binding protein 2 antibody, MIR3175 antibody, CHD2 antibody, Chd2 antibody

Background: Chromodomain-helicase-DNA-binding protein 2 is an enzyme that in humans is encoded by the CHD2 gene. The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 catalyzes the assembly of chromatin into periodic arrays, and the N-terminal region of CHD2, which contains tandem chromodomains, serves an auto-inhibitory role in both the DNA-binding and ATPase activities of CHD2.

Synonyms: Chromodomain-helicase-DNA-binding protein 2, CHD-2, ATP-dependent helicase CHD2, CHD2

Gene ID: 1106

UniProt: O14647