CAS9 antibody

Details for Product No. ABIN5526139, Supplier: Log in to see
Antigen
Reactivity
Streptococcus pyogenes (S. pyogenes)
8
1
Host
Mouse
5
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
9
4
3
3
3
2
1
Options
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Purpose Monoclonal antibody to detect Cas9.
Immunogen N-terminal fragment of Streptococcus pyogenes Cas9.
Clone 7A9
Isotype IgG1 kappa
Purification Affinity purified using protein G
Background Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. Cas9 is used to interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. This is accomplished by unwinding foreign DNA and checking for if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA.
Synonyms: CRISPR-associated endonuclease Cas9/Csn1, CRISPR associated protein 9, SpyCas9
Gene ID 901176
UniProt Q99ZW2
Application Notes Immunofluorescence: 1:500-1:2,000
Immunoprecipitation: 1:20
Western Blot: 1:1,000
Comment

Suggested positive control:
IF, IP, WB

Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use
Storage -20 °C
Expiry Date 12 months