IL13 Receptor alpha 1 antibody (Extracellular Domain)

Catalog No. ABIN5540413

Product Details anti-IL13 Receptor alpha 1 Antibody

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL13 Receptor alpha 1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Specificity: Clone GM1C8 recognises IL-13Rα1 transiently expressed on the cell surface of transfected BOSC cells as well as the native protein on monocytes (W. Luttmann, unpublished data). Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells. Species: Human.

Purification: Protein G chromatography

Immunogen: cDNA of the human IL-13Ralpha1 chain extracellular domain (U62858).

Clone: GM1C8

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 μg/10 6 cells. The antibody is routinely tested by on BOSC cells transiently transfected with an IL-13Rα1 expression vector. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. It is expected that the antibody will be suited for all applications where recognition of the native protein is required.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2 Preservatives: 0.01 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-IL13 Receptor alpha 1 antibody (ABIN5540413)

Bizzarro, Fontanella, Carratù, Belvedere, Marfella, Parente, Petrella: "Annexin A1 N-terminal derived peptide Ac2-26 stimulates fibroblast migration in high glucose conditions." in: PLoS ONE, Vol. 7, Issue 9, pp. e45639, (2013) (PubMed).

Thomas, Zhu, Schnaar, Alves, Konstantopoulos: "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15647-55, (2008) (PubMed).

Kunii, Nemoto, Kanaya, Tsubahara, Shimauchi: "Expression of CD13/aminopeptidase N on human gingival fibroblasts and up-regulation upon stimulation with interleukin-4 and interleukin-13." in: Journal of periodontal research, Vol. 40, Issue 2, pp. 138-46, (2005) (PubMed).

Bade, Lohrmann, ten Brinke, Wolbink, Wolbink, ten Berge, Virchow, Luttmann, Hack: "Detection of soluble human granzyme K in vitro and in vivo." in: European journal of immunology, Vol. 35, Issue 10, pp. 2940-8, (2005) (PubMed).

Target Details for IL13 Receptor alpha 1

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Alternative Name: cd213a1,il13ra1 (IL13RA1 Products)

Synonyms: CD213A2 antibody, CT19 antibody, IL-13R antibody, IL13BP antibody, CD213A1 antibody, IL-13Ra antibody, NR4 antibody, AI882074 antibody, CD213a1 antibody, IL-13r[a] antibody, Il13ra antibody, IL13RA1 antibody, il-13ra1 antibody, si:ch211-150g2.2 antibody, interleukin 13 receptor subunit alpha 2 antibody, interleukin 13 receptor subunit alpha 1 antibody, interleukin 13 receptor, alpha 1 antibody, interleukin 13 receptor, alpha 1-like antibody, IL13RA2 antibody, IL13RA1 antibody, Il13ra1 antibody, il13ra1 antibody, LOC100360218 antibody

Background: Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and im-mune responses. IL-13Rα1 together with IL-4Rα forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation.

UniProt: P78552

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response