EDNRB antibody (Extracellular Domain, N-Term)

Catalog No. ABIN5541800

Product Details anti-EDNRB Antibody

Target: EDNRB (Endothelin Receptor Type B (EDNRB))

Binding Specificity: Extracellular Domain, N-Term

Reactivity: Human, Dog, Monkey, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDNRB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects N-terminal extracellular domain of Endothelin B Receptor (Family: GPCR Subfamily: Endothelin).

Purification: Peptide Immunogen Affinity Chromatography

Immunogen: Synthetic 19 amino acid peptide from N-terminal extracellular domain of human EDNRB

Application Details

Application Notes: Western Blot. Immunocytochemistry. Immunohistochemistry on Paraffin Embedded Sections : 4-11 μg/mL Antigen Retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20 to -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-EDNRB antibody (ABIN5541800)

Tanaka, Sho, Takayama, Wakatsuki, Matsumoto, Migita, Ito, Hamada, Nakajima: "Endothelin B receptor expression correlates with tumour angiogenesis and prognosis in oesophageal squamous cell carcinoma." in: British journal of cancer, Vol. 110, Issue 4, pp. 1027-33, (2014) (PubMed).

Jones, Brockbank, Mobbs, Le Good, Soma-Haddrick, Heuze, Langham, Timms, Newham, Needham: "The orphan G-protein coupled receptor RDC1: evidence for a role in chondrocyte hypertrophy and articular cartilage matrix turnover." in: Osteoarthritis and cartilage, Vol. 14, Issue 6, pp. 597-608, (2006) (PubMed).

Target Details for EDNRB

Target: EDNRB (Endothelin Receptor Type B (EDNRB))

Alternative Name: endothelin b receptor (EDNRB Products)

Synonyms: ABCDS antibody, ET-B antibody, ET-BR antibody, ETB antibody, ETBR antibody, ETRB antibody, HSCR antibody, HSCR2 antibody, WS4A antibody, Ednra antibody, Etb antibody, ETR-b antibody, ETb antibody, Sox10m1 antibody, EDNRB antibody, abcds antibody, etb antibody, etrb antibody, hscr antibody, hscr2 antibody, endothelin receptor type B antibody, EDNRB antibody, Ednrb antibody, ednrb antibody

Background: The Endothelin Receptor ETB is expressed in most tissues. Mutations of the receptor gene are responsible for Hirschsprung disease type 2, a multigenic disorder with phenotypes such as bicolored irides, megacolon, hypopigmentation, and hearing loss. The ETB receptor has similar affinity for all three endothelins and activates a phosphatidylinositol-calcium second messenger system. A splice variant, termed SVR, has been described the sequence of the ETB-SVR receptor is identical to ET-B receptor, with the exception of the intracellular C-terminal domain. While both splice variants bind endothelin ET1, they exhibit different responses, which suggests that they may be functionally distinct. The endothelin B receptor has been reported to be expressed in adrenal, aorta, artery, brain, breast, eye, epididymis, ganglion, heart, kidney, liver, lung, ovary, placenta, prostate, skin, testis, uterus, and vessel.

UniProt: P24530

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process