KDM5D antibody (C-Term)

Catalog No. ABIN5541916

Product Details anti-KDM5D Antibody

Target: KDM5D (Lysine (K)-Specific Demethylase 5D (KDM5D))

Binding Specificity: AA 1248-1539, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM5D antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Human JARID1D. Other species not tested.

Purification: Protein A Chromatography

Immunogen: Highly pure (> 95%) recombinant Human JARID1D (Leu1248-Leu1539) fused to a C-terminal His-tag (6x His) derived from E. coli (Cat.-No AR60001PU-N)

Isotype: IgG

Application Details

Application Notes: Western blot: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Buffer: Purification: Protein A Chromatography

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for KDM5D

Target: KDM5D (Lysine (K)-Specific Demethylase 5D (KDM5D))

Alternative Name: jarid1d (KDM5D Products)

Synonyms: JARID1D antibody, Jarid1d antibody, DKFZp468I1010 antibody, HY antibody, HYA antibody, SMCY antibody, Smcy antibody, lysine demethylase 5D antibody, lysine (K)-specific demethylase 5D antibody, lysine demethylase 5C antibody, KDM5D antibody, Kdm5d antibody, KDM5C antibody

Background: H-Y antigen is defined as a male histocompatibility antigen that causes rejection of male skin grafts by female recipients of the same inbred strain of rodents. Male-specific, or H-Y antigen(s), are also detected by cytotoxic T cells and antibodies. H-Y antigen appears to be an integral part of the membrane of most male cells. In addition, H-Y antibodies detect a soluble form of H-Y that is secreted by the testis. The gene (Smcy/SMCY) coding for H-Y antigen detected by T cells has been cloned. It is expressed ubiquitously in male mice and humans, and encodes an epitope that triggers a specific T-cell response in vitro. Additional epitopes coded for by different Y-chromosomal genes are probably required in vivo for the rejection of male grafts by female hosts. The molecular nature of H-Y antigen detected by antibodies on most male cells is not yet known. Testis-secreted, soluble H-Y antigen, however, was found to be identical to Müllerian-inhibiting substance (MIS). MIS cross-reacts with H-Y antibodies and identical findings were obtained for soluble H-Y antigen and MIS, i.e., secretion by testicular Sertoli and, to a lesser degree, ovarian cells, binding to a gonad-specific receptor, induction of gonadal sex reversal in vitro and, in cattle, in vivo. H-Y antisera also detect a molecule or molecules associated with the heterogametic sex in non-mammalian vertebrates. Molecular data on this antigen or antigens are not yet available.

Pathways: Warburg Effect