This antibody recognizes CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Purification
Affinity Chromatography on Protein A
Immunogen
IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
IL3RA
Reactivity: Human, Mouse
WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry. Immunoprecipitation. Western blot. Immunohistochemistry on Frozen Sections.
Restrictions
For Research Use only
Format
Liquid
Buffer
95 % pure by SDS-PAGE) Purified
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Expiry Date
12 months
Martín-Gayo, Sierra-Filardi, Corbí, Toribio: "Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development." in: Blood, Vol. 115, Issue 26, pp. 5366-75, (2010) (PubMed).
Nazareth, Broderick, Simpson-Abelson, Kelleher, Yokota, Bankert: "Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5552-62, (2007) (PubMed).
Mayr, Bund, Schlee, Moosmann, Kofler, Hallek, Wendtner: "Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes." in: Blood, Vol. 105, Issue 4, pp. 1566-73, (2005) (PubMed).
Herling, Teitell, Shen, Medeiros, Jones: "TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin." in: Blood, Vol. 101, Issue 12, pp. 5007-9, (2003) (PubMed).
CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.