PPP4C antibody (C-Term)

Catalog No. ABIN452428

Product Details anti-PPP4C Antibody

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Binding Specificity: C-Term

Reactivity: Human, Rat, Dog, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP4C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: c-PQETRGIPSK KP

Specificity: This antibody recognizes PPP4C (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Canine, Rabbit and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography.

Immunogen: Peptide with sequence from the C-Terminus of Human PPP4C.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 35 kDa band observed in HEK293, A431 and HeLa lysates (calculatedMW of 35.5 kDa according to NP_002711.1). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Human Prostate shows nuclear staining ofsecretory cells inthe gland. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris-saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PPP4C

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Alternative Name: PPP4C (PPP4C Products)

Synonyms: pp4 antibody, ppx antibody, pp4c antibody, pph3 antibody, MGC75928 antibody, PP4C-A antibody, gc:56413 antibody, ppp4c antibody, wu:fd05e08 antibody, zgc:56413 antibody, PP4 antibody, PP4C antibody, PPH3 antibody, PPP4 antibody, PPX antibody, 1110002D08Rik antibody, AU016079 antibody, Ppx antibody, protein phosphatase 4 catalytic subunit antibody, protein phosphatase 4, catalytic subunit a antibody, protein phosphatase 4, catalytic subunit antibody, protein phosphatase 4 catalytic subunit S homeolog antibody, ppp4c antibody, ppp4ca antibody, PPP4C antibody, Ppp4c antibody, ppp4c.S antibody

Background: Protein phosphatase 4C may be involved in microtubule organization. It binds 1 iron ion and 1 manganese ion per subunit. PP4 consists of a catalytic subunit PPP4C and a regulatory subunit. PPP4R1 and belongs to the PPP phosphatase family, PP X subfamily.Synonyms: PP-X, PP4C, PPP4, PPX, Protein phosphatase X, Serine/threonine-protein phosphatase 4 catalytic subunit

Gene ID: 5531

NCBI Accession: NP_002711

UniProt: P60510