Ephrin B1 antibody (pTyr298)
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- Target See all Ephrin B1 (EFNB1) Antibodies
- Ephrin B1 (EFNB1)
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Binding Specificity
- pTyr298
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Reactivity
- Human, Mouse, Rat, Chicken, Zebrafish (Danio rerio), Cow, Dog, Xenopus laevis, Non-Human Primate
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Ephrin B1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Specific for the ~46k Ephrin-B protein phosphorylated at Tyr298. Immunolabeling of the Ephrin-B band is blocked by lambda-phosphatase treatment.
- Cross-Reactivity (Details)
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Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat. - Purification
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- Immunogen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr298 of Xenopus Ephrin-B.
- Isotype
- IgG
- Top Product
- Discover our top product EFNB1 Primary Antibody
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- Application Notes
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Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Target
- Ephrin B1 (EFNB1)
- Alternative Name
- Ephrin-B1 (EFNB1 Products)
- Synonyms
- EFNB1 antibody, LERK2 antibody, Cek5-L antibody, EFL-3 antibody, Elk-L antibody, Epl2 antibody, Eplg2 antibody, LERK-2 antibody, Lerk2 antibody, Stra1 antibody, CFND antibody, CFNS antibody, EFL3 antibody, EPLG2 antibody, cfnd antibody, cfns antibody, efl3 antibody, efnb1-A antibody, ephrinB1 antibody, eplg2 antibody, lerk2 antibody, ephrin B1 antibody, ephrin-B1 antibody, ephrin-B1 L homeolog antibody, EFNB1 antibody, Efnb1 antibody, efnb1 antibody, efnb1.L antibody
- Background
- Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2
- Gene ID
- 394292
- NCBI Accession
- NP_001080948
- UniProt
- O13097
- Pathways
- RTK Signaling
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