NOXO1 antibody (AA 238-252)

Catalog No. ABIN401322

Product Details anti-NOXO1 Antibody

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Binding Specificity: AA 238-252

Reactivity: Human, Chimpanzee, Dog, Guinea Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against NOXO1 protein. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from Human and Chimpanzee (100 % homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from Dog and Guinea Pig (87 %), as well as Rat (75 %) and Mouse (68 %). Reactivity against homologues from other sources is not known.

Purification: Immunoaffinity Chromatography.

Immunogen: Recombinant protein corresponding to amino acids 238-252 of Human NOXO1 protein

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody is tested for use in ELISA, Immunohistochemistry andWestern blot. Recommended Dilutions: ELISA: 1/2,0000-1/100,000.

Restrictions: For Research Use only

Handling

Concentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7. containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.

References for anti-NOXO1 antibody (ABIN401322)

Madrigal-Matute, Fernandez-Garcia, Gomez-Guerrero, Lopez-Franco, Muñoz-Garcia, Egido, Blanco-Colio, Martin-Ventura: "HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis." in: Cardiovascular research, Vol. 95, Issue 1, pp. 116-23, (2012) (PubMed).

Target Details for NOXO1

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Alternative Name: NOXO1 (NOXO1 Products)

Synonyms: NOXO1 antibody, cb18 antibody, sb:cb18 antibody, wu:fd09d09 antibody, noxo1 antibody, snx28 antibody, p41nox antibody, p41noxa antibody, p41noxb antibody, p41noxc antibody, sh3pxd5 antibody, P41NOX antibody, P41NOXA antibody, P41NOXB antibody, P41NOXC antibody, SH3PXD5 antibody, SNX28 antibody, 2310034C04Rik antibody, Snx28 antibody, hslt antibody, NADPH oxidase organizer 1 antibody, NOXO1 antibody, noxo1 antibody, noxo1.1 antibody, Noxo1 antibody

Background: The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells. NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region. NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5

Gene ID: 124056

NCBI Accession: NP_653204

UniProt: Q8NFA2