TRAF2 antibody (Internal Region)

Catalog No. ABIN401469

Product Details anti-TRAF2 Antibody

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody is directed against TRAF2 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region human TRAF2

Isotype: IgG

Application Details

Application Notes: ELISA: 1/60,000 - 1/250,000. Western Blot: 1 μg/m. Immunohistochemistry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.93 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for TRAF2

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Alternative Name: TRAF2 / TRAP3 (TRAF2 Products)

Synonyms: MGC:45012 antibody, TRAP antibody, TRAP3 antibody, TANK antibody, AI325259 antibody, TNF-receptor-associated factor 2 antibody, conjugal transfer protein TraF antibody, TNF receptor associated factor 2 antibody, TNF receptor-associated factor 2 antibody, Tnf receptor-associated factor 2 antibody, LOC661716 antibody, traF2 antibody, TRAF2 antibody, Traf2 antibody

Background: TRAF2, or Tumor Necrosis factor (TNF) Receptor-Associated Factor 2, is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Association to the receptor is also mediated by the interaction with TRADD. TRAF2 mediates activation of NF-kappa-B and MAPK8/JNK and is involved in apoptosis. TRAF2 forms a heterodimeric complex with TRAF1, which then recruits the inhibitor-of-apoptosis proteins (IAPs), apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 for the inhibition of caspase activation. In this way it functions as a mediator of the anti-apoptotic signals from TNF receptors. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. TRAF2 may be involved in IL-15 signaling. Multiple alternatively spliced transcript variants exist, but the biological validity of only one transcript has been determined.Synonyms: TNF receptor-associated factor 2, Tumor necrosis factor type 2 receptor-associated protein 3

Gene ID: 7186

NCBI Accession: NP_066961

UniProt: Q12933

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response, S100 Proteins