Apolipoprotein M antibody (C-Term)

Catalog No. ABIN452682

Product Details anti-Apolipoprotein M Antibody

Target: Apolipoprotein M (APOM)

Binding Specificity: C-Term

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Apolipoprotein M antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Apolipoprotein M (Apo M).

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PRNQEACELSNN, from the C Terminus of the protein sequence according to NP_061974.2.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/8000. Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Kidney andLiver lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 21.0 kDaaccording to NP_061974.2. We do note that ApoM is a glycoprotein which makes a highersize band to be expected. The 50 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3-6 μg/mL. In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Apolipoprotein M

Target: Apolipoprotein M (APOM)

Alternative Name: Apolipoprotein M (Apo M) (APOM Products)

Synonyms: G3a antibody, HSPC336 antibody, NG20 antibody, apo-M antibody, 1190010O19Rik antibody, APOM antibody, fb62d12 antibody, wu:fb62d12 antibody, g3a antibody, ng20 antibody, hspc336 antibody, mKIAA4033 antibody, apolipoprotein M antibody, apolipoprotein M S homeolog antibody, myeloperoxidase antibody, APOM antibody, Apom antibody, apom.S antibody, apom antibody, Mpo antibody

Background: Apolipoprotein M is a member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. It is positively related to leptin. It is mainly expressed in liver and kidney. Apolipoprotein M is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions.Synonyms: Apo-M, ApoM, HSPC336, NG20, Protein G3A

Gene ID: 55937

NCBI Accession: NP_061974

UniProt: O95445