PRKD3 antibody (C-Term)

Catalog No. ABIN359072

Product Details anti-PRKD3 Antibody

Target: PRKD3 (Protein Kinase D3 (PRKD3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody reacts to PKC nu.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Immunofluorescence: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PRKD3

Target: PRKD3 (Protein Kinase D3 (PRKD3))

Alternative Name: PRKD3 (PRKD3 Products)

Synonyms: EPK2 antibody, PKC-NU antibody, PKD3 antibody, PRKCN antibody, nPKC-NU antibody, PRKD3 antibody, 4930557O20Rik antibody, 5730497N19Rik antibody, Pkcnu antibody, Prkcn antibody, protein kinase D3 antibody, PRKD3 antibody, prkd3 antibody, Prkd3 antibody

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.Synonyms: EPK2, PKC nu, PKC-nu, PRKCN, Protein kinase C nu type, Serine/threonine-protein kinase D3, nPKC-nu

Gene ID: 23683, 9606

UniProt: O94806