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SIAH1 antibody (N-Term)

SIAH1 Reactivity: Human, Rat, Mouse, Dog, Cow WB, IP, EIA Host: Goat Polyclonal unconjugated
Catalog No. ABIN374155
  • Target See all SIAH1 Antibodies
    SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
    Binding Specificity
    • 15
    • 7
    • 7
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 37
    • 25
    • 24
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Human, Rat, Mouse, Dog, Cow
    Host
    • 48
    • 2
    • 2
    Goat
    Clonality
    • 50
    • 2
    Polyclonal
    Conjugate
    • 20
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIAH1 antibody is un-conjugated
    Application
    • 43
    • 23
    • 13
    • 13
    • 6
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Sequence
    RQTATALPTG TSKC
    Specificity
    This product is expected to recognize both reported isoforms (according to NP_003022.3, NP_001006611.1).
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse, Rat, Canine (Dog), and Cow.
    Species reactivity (tested):Human.
    Purification
    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
    Immunogen
    Peptide from N-Terminus of the protein sequence according to NP_003022.3, NP_001006611.1 Genename: SIAH1
    Top Product
    Discover our top product SIAH1 Primary Antibody
  • Application Notes
    Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 28+30 kDa bands observed in Human Liver lysates(calculated MW of 31.0 kDa and 34.0 kDa according to NP_003022.3 and NP_001006611.1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Donati, Marseglia, Magi, Serratì, Cencetti, Bernacchioni, Nannetti, Benelli, Brunelli, Torricelli, Cossu, Bruni: "Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6." in: PLoS ONE, Vol. 6, Issue 5, pp. e20389, (2011) (PubMed).

  • Target
    SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
    Alternative Name
    SIAH1 (SIAH1 Products)
    Synonyms
    SIAH antibody, Siah1 antibody, Siah1a antibody, id:ibd2635 antibody, zgc:56026 antibody, SIAH1A antibody, siah antibody, E3 ubiquitin-protein ligase Siah1 antibody, E3 ubiquitin-protein ligase siah-1 antibody, siah E3 ubiquitin protein ligase 1 antibody, siah E3 ubiquitin protein ligase 1 L homeolog antibody, Tsp_03055 antibody, siah-1 antibody, Siah1 antibody, siah1 antibody, SIAH1 antibody, siah1.L antibody
    Background
    Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1 , both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. GAPDH may be genetically associated with late-onset Alzheimer's disease. (-)-deprenyl, which has been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and has neuroprotective actions. The molecular action of this protection is as yet not clear.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a
    Gene ID
    6477
    NCBI Accession
    NP_003022
    UniProt
    Q8IUQ4
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