KLF8 antibody (N-Term)

Catalog No. ABIN374228

Product Details anti-KLF8 Antibody

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VDMDKLINNL EVQ-C

Specificity: This antibody detects KLF8 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_009181.2, NP_001152768.1 Genename: KLF8

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in Human Kidney lysates (calculatedMW of 39.3 acording to NP_009181.2). In glioblastomas this protein was reported to run atappr.60 kDa (Ding et al . J Biol Chem.2005 Feb 25,280(8): 6802-15, PMID: 15557280).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for KLF8

Target: KLF8 (Kruppel-Like Factor 8 (KLF8))

Alternative Name: KLF8 (KLF8 Products)

Synonyms: klf8 antibody, BKLF3 antibody, ZNF741 antibody, A830097P10Rik antibody, ZNF74 antibody, Klf8-ps1 antibody, RGD1565831 antibody, Kruppel like factor 8 antibody, Kruppel-like factor 8 antibody, KLF8 antibody, klf8 antibody, Klf8 antibody

Background: Kruppel-like factor proteins all contain a characteristic C-terminal DNA binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. KLF8 is a transcriptional repressor and activator. It also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). Ubiquitously expressed.Synonyms: BKLF3, Basic krueppel-like factor 3, KLF8, Krueppel-like factor 8, ZNF7, Zinc finger protein 741

Gene ID: 11279

NCBI Accession: NP_009181

UniProt: O95600