MRPL3 antibody (C-Term)

Catalog No. ABIN374279

Product Details anti-MRPL3 Antibody

Target: MRPL3 (Mitochondrial Ribosomal Protein L3 (MRPL3))

Binding Specificity: C-Term

Reactivity: Human, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This MRPL3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Sequence: NVCQPGAPSI TFA

Specificity: This antibody detects MRPL3 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Pig, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_009139.1 Genename: MRPL3

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of cell line HepG2(calculated MW of 38.5 kDa according to NP_009139.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for MRPL3

Target: MRPL3 (Mitochondrial Ribosomal Protein L3 (MRPL3))

Alternative Name: MRPL3 (MRPL3 Products)

Synonyms: COXPD9 antibody, MRL3 antibody, RPML3 antibody, GB13776 antibody, 2010320L16Rik antibody, 5930422H18Rik antibody, AA407889 antibody, mrpl3 antibody, fj61d01 antibody, wu:fj61d01 antibody, zgc:66423 antibody, mitochondrial ribosomal protein L3 antibody, 39S ribosomal protein L3, mitochondrial antibody, mitochondrial 54S ribosomal protein YmL3 antibody, mitochondrial ribosomal protein L3 S homeolog antibody, MRPL3 antibody, LOC552676 antibody, Mrpl3 antibody, mrpl3.S antibody, mrpl3 antibody

Background: Mammalian mitochondrial ribosomal proteins (MRPs) are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This antibody targets a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q.Synonyms: 39S ribosomal protein L3, L3mt, MRL3, MRP-L3, RPML3, mitochondrial, mitochondrial 60S ribosomal protein L3

Gene ID: 11222

NCBI Accession: NP_009139

UniProt: P09001