BAZ2B antibody (C-Term)

Catalog No. ABIN374295

Product Details anti-BAZ2B Antibody

Target: BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Cow, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This BAZ2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-YFEKKWTDTF KVS

Specificity: This antibody detects BAZ2B at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for BAZ2B

Target: BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))

Alternative Name: BAZ2B (BAZ2B Products)

Synonyms: BAZ2B antibody, LOC100229551 antibody, WALp4 antibody, 5830435C13Rik antibody, BC053917 antibody, D2Ertd794e antibody, bromodomain adjacent to zinc finger domain 2B antibody, bromodomain adjacent to zinc finger domain, 2B antibody, BAZ2B antibody, baz2b antibody, Baz2b antibody

Background: BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4

Gene ID: 29994

NCBI Accession: NP_038478

UniProt: Q9UIF8