AKAP3
Reactivity: Human
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 90 kDa band observed in Human Testis lysates (calculatedMW of 90 kDa according to NP_006413). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. AKAP3 belongs to the AKAP110 family. Expression is testis specific, and is only expressed in spermatids. It is thought to function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.Synonyms: A-kinase anchor protein 110 kDa, A-kinase anchor protein 3, AKAP110, Cancer/testis antigen 82, Fibrous sheath protein of 95 kDa, Fibrousheathin I, Fibrousheathin-1, PRKA3, Protein kinase A-anchoring protein 3, SOB1, Sperm oocyte-binding protein