SERPINI2 antibody (Internal Region)

Catalog No. ABIN374538

Product Details anti-SERPINI2 Antibody

Target: SERPINI2 (serpin Peptidase Inhibitor, Clade I (Pancpin), Member 2 (SERPINI2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SERPINI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is expected to recognize both reported isoforms (NP_006208.1 and NP_001012303.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine and (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KGDWKQKFRKEDTQ, from the internal region of the protein sequence. Genename: SERPINI2

Application Details

Application Notes: Peptide ELISA: Antibody detection limit dilution: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 40 kDa band observed in Human Pancreas lysates (calculated MW of 46.0 kDaaccording to NP_006208.1 and 47.0 kDa and NP_001012303.1). Immunohistochemistry on Paraffin Sections: 3 μg/mL. In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and ofrare cells in the islets of Langerhans.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preserative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SERPINI2

Target: SERPINI2 (serpin Peptidase Inhibitor, Clade I (Pancpin), Member 2 (SERPINI2))

Alternative Name: SERPINI2 (SERPINI2 Products)

Synonyms: Spi14 antibody, MEPI antibody, PANCPIN antibody, PI14 antibody, TSA2004 antibody, serpin family I member 2 antibody, serpin peptidase inhibitor, clade I (pancpin), member 2 L homeolog antibody, serpin peptidase inhibitor, clade I (pancpin), member 2 antibody, serine (or cysteine) peptidase inhibitor, clade I, member 2 antibody, SERPINI2 antibody, serpini2.L antibody, Serpini2 antibody

Background: MEP1 is a member of the serine protease inhibitor (serpin) superfamily made up of proteins which play central roles in the regulation of a wide variety of physiological processes, including coagulation, fibrinolysis, development, malignancy and inflammation. It may have a role in a growth-control, possibly growth-suppressing pathway and, when impaired, may be involved in pancreatic carcinogenesis. The protein is a member of the plasminogen activator inhibitor-1 family, a subset of the serpin superfamily whose members act as tissue-specific tPA inhibitors. Two alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.Synonyms: MEPI, Myoepithelium-derived serine protease inhibitor, PI14, Pancpin, Protease inhibitor 14, TSA2004

Gene ID: 5276

NCBI Accession: NP_006208

UniProt: O75830